Wednesday, April 6, 2022 - Thesis defense - Arthur BEAUCHET

"Functional characterization of FW2.2, a plasmodesmata located protein"


Team Flowering, Fruit Development and Environmental Constraints (FDFE)

Wednesday, April 6, 2022- 2 p.m. - Amphitheater Colette & Josy Bové - Campus INRAE Villenave d'Ornon

Abstract :

FW2.2 was identified first as a major QTL controlling for up to 30% of the fruit weight variation. FW2.2 is a negative regulator of cell division but its function remains unclear. Here, gain- and loss-of-function plants and GUS reporter lines under FW2.2 promoter were generated to investigate its mode of action. We demonstrated that FW2.2 is localised to plasmodesmata, a channel that provide direct cytosolic continuity between two cells. Gain-of-function plant show an increase in cell-to-cell diffusion in leaf. Level of callose deposition was measured at two different developmental stages of fruit development and an increase of callose was reported in loss-of-function plant.  On the contrary, level of callose deposition decrease when FW2.2 is overexpressed suggesting a role of FW2.2 in callose deposition/degradation to control cell-to-cell communication.

Jury :

  • M. Christian CHEVALIER
    Directeur de Recherche Université de Bordeaux - UMR BFP INRAE Bordeaux - Directeur de Thèse
    Associate Professor University of Leeds - Faculty of Biological Sciences - Rapporteur
  • Mrs Esther VAN DER KNAAP
    Professor University of Georgia, Department of Horticulture - Rapporteur
  • M. Sébastien MONGRAND
    Directeur de Recherche CNRS - LBM campus INRAE Bordeaux - Examinateur
  • M. Jérôme JOUBES
    Directeur de Recherche CNRS - LBM campus INRAE Bordeaux - Examinateur
  • Mrs Françoise MONEGER
    Directrice de Recherche ENS de Lyon - Examinateur

Modification date : 17 October 2023 | Publication date : 17 March 2022 | Redactor : M. Gauthier